Sandwich cultured hepatocytes
Webb10 feb. 2024 · Eventually, artificial cell sheets of cultured skin were created, targeting various tissues and organs, including epithelial sheets, chondrocyte sheets, and myoblast cell sheets. ... function, and physiological responsiveness: Hepatocytes cultured in a sandwich configuration. FASEB J. 1996, 10, 1471–1484. [Google Scholar] Webb1 maj 2024 · As sandwich-cultured hepatocytes (SCHs) maintain metabolic activities and form a canalicular network, the whole interplay between uptake and efflux transporters …
Sandwich cultured hepatocytes
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WebbHepatocytes were isolated after perfusion and enzymatic disaggregation, and were first maintained in hormonally defined media on a Matrigel matrix, and then transferred to collagen sandwich gel. WebbMarion TL, L 1. eslie EM and Brouwer, KL (2007) Use of sandwich-cultured hepatocytes to evaluate impaired bile acid transport as a mechanism of drug-induced hepatotoxicity. Mol Pharm 4:911-918. Soars MG, Grime K, Sproston JL, Webborn PJ and Riley, RJ (2007) Use of hepatocytes to assess the contribution of hepatic uptake to clearance in vivo.
Webb26 apr. 2024 · The maintenance of terminally differentiated cells, especially hepatocytes, in vitro has proven challenging. Here we demonstrated the long-term in vitro maintenance of primary human hepatocytes (PHHs) by modulating cell signaling pathways with a combination of five chemicals (5C). 5C-cultured PHHs showed global gene expression … Webb26 mars 2013 · Typically, hepatocytes have been cultured in systems that allowed cell attachment on one surface and medium on the opposite surface [1,2]. There are many …
WebbSandwich-cultured hepatocyte services Overview Related Services Qualyst Transporter Certified hepatocytes Primary hepatocyte cultures can vary widely in terms of quality … Webbcells cultured on amorphous collagen I between days 2 and 13, and the gradual loss in the number of cells cultured in collagen I gels. In contrast, hepatocytes cultured on Corning Matrigel Matrix maintain a constant cell number through day 22. 0.0 0.2 0.4 0.6 0.8 1.0 12 58 13 16 17 19 22 Days in Culture
Webb30 okt. 2013 · The objective of this study was to compare the metabolism of T4 in untreated and 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153)-treated primary sandwich-cultured hepatocytes from rat (SCRH) and human ...
Webb30 jan. 2014 · Sandwich-cultured human hepatocytes were used to study the hepatobiliary distribution and accumulation of exogenous taurocholate, ERAs and endogenous bile acids. The molecular mechanisms for findings in hepatocytes or clinical observations were further explored using either vesicular assays (efflux transporters) or transfected cell … navy army mortgage center corpus christiWebbSandwich-cultured hepatocytes maintain expression of liver-specific proteins and gradually form intact bile canaliculi with functional biliary excretion of endogenous … navy army mission texasWebb30 sep. 2014 · Clinical DILI can be predicted with a sensitivity of ~50% and a false positive (FP) rate of ~5% using 24-h cultures of sandwich-cultured primary human hepatocytes and imaging of four cell injury ... markham marriott courtyardWebb23 nov. 2012 · Even though phase I enzyme gene expression decreases over time and phase II enzymes remain expressed at relatively high levels, data indicate that P450 gene expression in sandwich cultured mouse hepatocytes is still more stable than in monolayers of primary hepatocytes. 53 It has been suggested that the sandwich culture model is … navy army mortgage paymentWebb1 apr. 2024 · Primary human hepatocytes (PHHs) are commonly used for in vitro studies of drug-induced liver injury. However, when cultured as 2D monolayers, PHH lose … markham mayor\u0027s youth councilWebbSandwich-cultured human hepatocytes were used to study the hepatobiliary distribution and accumulation of exogenous taurocholate, ERAs and endogenous bile acids. The mol... Cite Similar... markham mayor election 2022Webb7 mars 2013 · Sandwich-cultured hepatocytes are a promising cellular model . In our previous study, the rates of metabolite formation are much lower in conventional primary hepatocyte culture models than in the organotypical model [ 20 ]. markham massage therapist