2024 Peasy‐uni seamless cloning and assembly kit

Peasy‐uni seamless cloning and assembly kit

Author: hcve

August undefined, 2024

WebSeamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of... WebFeatures of the pEASY-Uni Seamless Cloning and Assembly Kit Fast assembly and fast cloning: 15 minutes Easy: regardless of restriction enzyme sites of inserts, and …

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WebTransGen Biotech pEASY® -Uni Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional … WebSep 29, 2024 · The fragments were ligated using the pEASY-Uni Seamless Cloning and Assembly Kit, and the constructed plasmid was transformed into AsCEP112 mutant protoplasts using PEG-mediated protoplast transformation technology. Finally, neomycin resistance was used to screen for revertant strains. Melanin, penetration and … integral on calc on ti-84

Identification of effector CEP112 that promotes the infection of ...

WebBut, if assembly by OE-PCR is used to put together fragments in groups of 3, then seamless DNA assembly using pEASY-Uni will become easy enough to get our clones rapidly. We use the Fast & Steep PCR protocol to accomplish this. pEASY®-Uni Seamless Cloning and Assembly Kit Easily assemble up to 5 DNA fragments with CU101 pEASY-Uni Web5 rows · Product Details. This kit takes advantage of proprietary recombinases and homologous recombination ... WebNov 25, 2024 · They were all constructed using the pEASY-Uni Seamless Cloning and Assembly Kit (TransGene Biotech, China), which can be used to recombine the vector linearized by any method and the PCR fragment with 15–25bp overlapping region with both ends of the linearized vector. jockey c todd

In-Fusion™ assembly: seamless engineering of multidomain …

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WebAug 2, 2024 · Evaluation of pEASY-Uni Seamless Cloning and Assembly Kit to clone multiple fragments of Elaeis guineensis DNA. Meta Gene, Vol. 14. A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector. 28 February 2024 BMC Biotechnology, Vol. 17, No. 1. WebMay 17, 2016 · The PQQ and terminal ubiquinol cytochrome bo 3 oxidase fused expression plasmid was constructed by the pEASY-Uni seamless cloning and assembly kit CU101 (TransGen, China). ... The cDNA was synthesized from the total RNA using a PrimeScript RT Reagent Kit (Perfect Real Time) (Takara) according to the manufacturer’s protocol. integral orthogonal matrixWebApr 12, 2024 · The subsequent ligation reaction was carried out at 50 °C according to the pEASY-Basic Seamless Cloning and Assembly Kit (TransGen Biotech), after which ligation products were transformed into E. coli DH5α. Positive colonies were screened and plasmid was isolated and sent to Sangon (Shanghai, China) for DNA sequencing. Protein purification integral optical density iod

"WebNov 30, 2024 · According to the protocol of the pEASY ®-Uni Seamless Cloning and Assembly Kit (TransGen Biotech), the ORFs of the targeted TwFTase / GGTase I-α and TwFTase-β were inserted into the Kpn I site of the pCAMBIA1300-EGFP vector (Figure S1), which carries the eGFP gene driven by the 35S promoter (the vector was supplied by Prof. … " - Peasy‐uni seamless cloning and assembly kit

Peasy‐uni seamless cloning and assembly kit

GeneArt™ Seamless Cloning and Assembly Kit - Thermo …

WebOct 13, 2024 · To increase protein solubility, four TwCPR ORFs were subcloned into the N-terminal MBP fusion expression vector HIS-MBP-pET28a according to the protocol of the pEASY® -Uni Seamless Cloning and Assembly Kit (TransGen Biotech; HIS-MBP-pET28a was provided by Dr. Xiaohong Zhang. HIS, histidine; MBP, maltose-binding protein. WebSep 1, 2024 · The PCR products were then transformed by use of pEASY-Uni Seamless Cloning and Assembly Kit (Moradpour and Abdulah 2024), into pBI121-GFP vector …

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WebSep 24, 2024 · The pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech) was used to construct different recombinogenic DNA fragments according to the … WebThermo Fisher peasy uni seamless cloning assembly kit Peasy Uni Seamless Cloning Assembly Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: …

WebpEASY® -Uni Seamless Cloning and Assembly Kit 目录号： CU101-01 单价：￥910 规格： 10 rxns 20 rxns 60 rxns 数量： - + 说明书质检报告立即下单加入购物车产品详情介绍本 … WebMay 16, 2024 · The junction between two pieces of DNA can be made seamless by including no additional DNA sequence. Alternatively, short pieces of DNA such as restriction sites, translation initiation sites, linkers, or epitope tags can be …

Webexperiments were performed with pEASY®-Uni Seamless Cloning and assembly kit (TransGen Biotech, Beijing, China). Construction of formolase error-prone (epPCR) library and screening epPCR libraries were generated by the standard epPCR method using variant formolase (fls) gene as template (21). For the mutagenic PCR (95 ºC for 2 min, 1 cycle ... WebNov 25, 2024 · They were all constructed using the pEASY-Uni Seamless Cloning and Assembly Kit (TransGene Biotech, China), which can be used to recombine the vector linearized by any method and the PCR fragment with 15–25bp overlapping region with both ends of the linearized vector. All the fragments needed for chloroplast expression vector …

WebJan 17, 2024 · Gibson Assembly® Master Mix – Assembly (E2611) v2 March 2024 New England Biolabs This protocol explains methods for the Gibson Assembly using the Gibson Assembly® Master Mix (E2611)....

WebDec 1, 2024 · pEASY-Uni Seamless Cloning and Assembly Kit was derived from the Gibson Assembly method with similar enzyme mixes. The advantages of pEASY -Uni Seamless … jockey crew neck jockey craig atkinsonWebDec 1, 2024 · The recombinant plasmid pPIC3.5 k-17β-HSD3 was constructed according to the method of pEASY-Uni Seamless Cloning and Assembly Kit. The zwf1 gene (Gene ID: 54633129) was amplified from Saccharomyces cerevisiae BY4741, and then ligated to the pPICZαA plasmid. jockey craig williamsWebpEASY®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products. Pricing & Availability Download Catalogue Add to wishlist. ... pEASY® -Uni Seamless Cloning and Assembly Kit. No restriction enzyme digestions, > 95% cloning efficiency, and no extra sequences introduced. jockey crossword clueWebTransGen Biotech pEASY®-Basic Seamless Cloning and Assembly Kit takes advantage of proprietary recombinases and homologous recombination to achieve directional … jockey crosswordWebDNA fragments were PCR amplified with Q5 DNA polymerase, purified using the EZ-10 Spin Column PCR Products Purification Kit (Bio-Basic, Canada) and assembled either using the pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech, China), following manufacturer’s instructions, or by E. coli mediated assembly [21]. jockey crewWebFeatures of the pEASY-Basic CU201. Fast assembly and fast cloning: 15 minutes. Easy: regardless of restriction enzyme sites of inserts, and elimination of restriction digestion … integral over a ball