2024 Expression of ascorbate oxidase

Expression of ascorbate oxidase

Author: ojle

August undefined, 2024

WebApr 26, 2024 · Ascorbate oxidase (AO) is a glycoprotein and a member of blue copper oxidase family that mediates ascorbate oxidation to dehydroascorbate (DHA) which usually follows high affinity transport across the membrane ( Smirnoff, 2000; Szarka et al., 2004; Barbehenn et al., 2008; De Tullio et al., 2013 ). WebJan 14, 2024 · The activities and expression of NADPH oxidase (NOX) and antioxidant enzymes in the corresponding samples were also determined. ... The total antioxidant capacity and the activities of antioxidant enzymes ascorbate peroxidase (APX), superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were measured using an …

A mutation affecting ASCORBATE PEROXIDASE 2 gene …

WebApr 13, 2024 · Here, localization of NPF8.4 in the tonoplast, together with the reduced vacuolar glycerate content displayed by an npf8.4 mutant and the glycerate efflux activity detected in an oocyte expression ... marriott new headquarters address

JMSE Free Full-Text Control of Reactive Oxygen Species through ...

WebMar 7, 2024 · Further experiments revealed that VvBKI1 interacts with a cytoplasmic ascorbate peroxidase, VvAPX1, an ROS-scavenging protein. Transient expression of VvAPX1 in grape and N. benthamiana promoted its resistance against P. viticola, and P. capsici. Moreover, VvAPX1 transgenic Arabidopsis is more resistant to H. arabidopsidis. Web30). Ascorbate oxidase is unlikely to be involved in scavenging activity because the reaction consumes molecular oxygen, rather than hydrogen peroxide. It is of interest in this respect to note that ascorbate-dependent peroxidase activity has been reported in the extracellular fluid of Phaseolus vulgaris leaves (34). WebAscorbate peroxidase (APX), reported first time in horseradish plant (1979), is an important enzyme known for higher affinity for intracellular H2 O 2 than other types of peroxidases like catalases, peroxiredoxins in Leishmania and Trypanosoma parasites. marriott new bedford ma

Kinase Inhibitor VvBKI1 Interacts with Ascorbate Peroxidase …

Webgenes whose expression changes in response to high light (HL), including the H 2O 2 scavenger, ASCORBATE PER-OXIDASE 2 (APX2). We carried out a screen in Arabi-dopsis thaliana for lesions that alter HL-induced expression of APX2 to identify components in abiotic stress signalling pathways. High light was used as it can be instan- WebNov 27, 2010 · AO is a cell wall-localized enzyme that uses oxygen to catalyze the oxidation of ascorbate to the unstable MDHA which rapidly disproportionates to yield DHA and AsA, and thus contributes to the modulation of the AsA redox state and perception of external and internal stimuli of plant cells. marriott newcastle under lymeWebOver-expression of ascorbate oxidase in the apoplast of transgenic tobacco results in altered ascorbate and glutathione redox states and increased sensitivity t Transgenic tobacco ( Nicotiana tabacum L. cv. Xanthi) plants expressing cucumber ascorbate oxidase (EC.1.10.3.3) were used to examine the role of extracellular ascorbic acid in ... marriott new atlanta

"WebSep 22, 2006 · Ascorbate oxidase (AO) is a cell wall-localized enzyme that uses oxygen to catalyse the oxidation of ascorbate (AA) to the unstable radical monodehydroascorbate (MDHA) which rapidly disproportionates to yield dehydroascorbate (DHA) and AA, and thus contributes to the regulation of the AA redox state. " - Expression of ascorbate oxidase

Expression of ascorbate oxidase

Ascorbic Acid-A Potential Oxidant Scavenger and Its Role in Plant ...

WebMar 20, 2024 · Ascorbate oxidase (AO) is a member of the multi-copper oxidase family of enzymes and converts ascorbate to monodehydroascorbate at the same time as reducing oxygen to water. The enzyme is present in the apoplastic space of plant cells and seems to be particularly highly expressed in roots and fruits of the Cucurbitaceae family. WebAscorbate oxidase is a copper-containing enzyme found in melons, seeds, cereals, and fruits and vegetables. It can oxidize ascorbic acid to produce water and dehydroascorbic acid. Compared with non-enzymatic oxidation, ascorbic acid oxidase acts to generate water, while the former generates hydrogen peroxide. Figure 1. Ascorbate oxidase dimer.

Did you know?

WebSep 6, 2016 · As a member of a small multigene family of multicopper oxidases, ascorbate oxidase (AO; EC 1.10.3.3) catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbate (DHA), thereby generating oxidative signals in apoplasts [6–8]. WebJul 13, 2024 · The homolog of laccase, ascorbate oxidase , was confirmed to be involved in cell growth and may control the cell-division cycle . SKU ... Kato, N.; Esaka, M. Changes in ascorbate oxidase gene expression and ascorbate levels in cell division and cell elongation in tobacco cells. Physiol. Plant 2010, 105, 321–329.

WebA thylakoidal APX (TAPX) gene was cloned from sugarcane leaves by using RT-PCR technique, and real-time fluorescence quantitative PCR analysis showed the TAPX gene expressed in the roots, stems and leaves of Sugarcane plant, and the highest expression level was found in the leaves. 8 References SHOWING 1-10 OF 20 REFERENCES … WebThe function of ascorbate oxidase in tobacco The function of the apoplastic enzyme ascorbate oxidase (AO) was investigated in tobacco (Nicotiana tabacum). The abundance of AO mRNA was up-regulated by light. Cytosolic ascorbate peroxidase (APX1) transcripts were also highest in the light. In contrast, L-galactono-gamma-lactone dehydrogenase, st …

WebMar 25, 2024 · Thermographic correlation analysis showed that the correlation between relative expression and log2 fold change values at 24 h, 48 h, 72 h and 96 h were very high, with R2 values of 0.96, 0.90, 0. ... WebA cDNA clone for ascorbate oxidase (AAO) has been isolated from a cDNA library of tobacco ( Nicotiana tabacum) cells. The identity of the amino acid sequence deduced from tobacco AAO cDNA to that from pumpkin AAO cDNA was 68%, which was much lower than the identity (80%) between pumpkin and cucumber AAO.

WebMay 1, 2003 · Over-expression of AO resulted in no change in the total ascorbate content recovered in apoplast washing fluid, but the redox state of ascorbate was reduced from 30% in wild-type leaves to below ...

WebAscorbate oxidase is a homodimeric enzyme with a molecular mass of 70 kDa and 552 amino acid residues per subunit (zucchini). The three-domain structure and the location of the type-1 and trinuclear copper centers in the ascorbate oxidase monomer as derived from the crystal structure are shown in Figure 33. marriott newcastle gatesheadWebSep 1, 2011 · AO expression levels in transgenic lines were monitored by semi-quantitative reverse transcriptase-polymerase chain reaction. The decreased AO enzyme activity and significantly improved AsA... marriott newcastle metroWeb57 rows · Ascorbate peroxidase (APX, EC 1.11.1.11) is a heme-peroxidase enzyme that utilizes ascorbate as a substrate to decompose H 2 O 2 into water and … marriott new castle nhWebJan 26, 2024 · Ascorbic acid is considered to be one of the most important antioxidants in plants and plays a vital role in the adaptation of plants to unfavorable conditions. In the present study, an ascorbate oxidase gene (Solyc04g054690) was over-expressed in cherry tomato cv. West Virginia 106 lines and compared with previously studied RNAi silenced … marriott newcastle gateshead metro centreWebFeb 17, 2024 · An enzyme named ascorbate oxidase (AAO) is known to oxidize ascorbate and deleteriously affect the plant system in response to stress. Genome-wide analysis of ascorbate oxidase gene family has led to the identification of five, three, seven, four and six AAO genes in Oryza sativa, Arabidopsis, Glycine max, Zea mays and Sorghum bicolor … marriott new hampshire coastWebApr 9, 2024 · HIGHLIGHTS. who: Stefania Fortunato et al. from the Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, Bari, Italy have published the paper: Redox Signaling in Plant Heat Stress Response, in the Journal: Antioxidants 2024, 12, 605. of /2024/ SUMMARY marriott new braunfels texasIn enzymology, a L-ascorbate oxidase (EC 1.10.3.3) is an enzyme that catalyzes the chemical reaction 2 L-ascorbate + O2 2 dehydroascorbate + 2 H2O Thus, the two substrates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. marriott newcastle uk