Cycloheximide protein stability assay
WebHere, a recently developed cellular thermal shift assay (CETSA) that is typically used to demonstrate protein-ligand binding was adapted to detect shifts in protein stabilization upon increasing O-GlcNAcylation levels in Nod2. WebAug 26, 2024 · The evaluation of protein stability was performed by the addition of 100 μg/mL cycloheximide (final concentration) (Sigma-Aldrich) to the induced cell cultures. Cell cultures (10 mL) were collected and pelleted upon the addition of cycloheximide, and thereafter at 30-min intervals until 180 min [ 27 ].
Cycloheximide protein stability assay
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WebJan 15, 2024 · Promoter-based luciferase assays further confirmed that, ... To understand if the decreased AGR2 by MG132 is caused by protein stability, cycloheximide (CHX), a protein synthesis inhibitor, was ... WebJan 5, 2015 · Analysis of protein stability was measured by the cycloheximide chase assay, slightly modified from (81). Parental wildtype or Traf3 -/-2B4 cells J o u r n a l P r …
WebApr 10, 2024 · To do so, we performed an immunoprecipitation assay followed by mass spectrometry (MS) to screen potential DUBs interacting with β-TrCP (Figure 1F and Figure S1B, Supporting Information). Through this method, we identified nine DUBs as potential regulators of β-TrCP protein stability (Figure S1C, Supporting Information). WebNov 10, 2024 · Protein stability assays. After 24 h of culture in a 6-well plate, cycloheximide (CHX, Sigma-Aldrich) was added to each well at a concentration of 50 …
WebApr 12, 2024 · Cycloheximide assay. HLF cells were transfected for 24 h with siUSP1 or siControl. ... we further evaluated TAZ protein stability in HLF cells with USP1 silencing and found that USP1 depletion ... WebJan 15, 2024 · To understand if the decreased AGR2 by MG132 is caused by protein stability, cycloheximide (CHX), a protein synthesis inhibitor, was included in the study …
WebNov 10, 2024 · Protein stability assays After 24 h of culture in a 6-well plate, cycloheximide (CHX, Sigma-Aldrich) was added to each well at a concentration of 50 µg/mL. For the PANC-1 cells transfected with sgVector or sgRNA A1×B2 plasmids, the protein was isolated after 0, 2, 4, 8 or 10 h of CHX treatment.
WebApr 4, 2024 · www.ncbi.nlm.nih.gov can india qualify for fifaWebOct 8, 2024 · The Duolink® In Situ Red Starter Kit Mouse/Rabbit for proximity ligation assay (PLA) were purchased from Sigma (DUO92101). The PLA was carried out according to the instructions on the kit. ... We investigated the effect of O-GlcNAc levels on c-Myc protein stability by using cycloheximide (CHX, 40325ES03, Yeasen Biotechnology), which is a ... can indices be decimalsWebComparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational … five9 and salesforceWebJul 27, 2015 · Protein stability assay. HeLa and NSC34 cells were co-transfected with equal amount of pcDNA4/a-SMN and pcDNA4/FL-SMN. After 20 hrs cells were treated with 100 μg/ml cycloheximide (CHX, Calbiochem, Darmstadt, Germany) to inhibit protein synthesis. ... or cycloheximide, to block protein translation and allow mRNA … five9 api referenceWebJan 31, 2024 · Here, we demonstrate that hnRNP-E1 protein stability is increased upon ARIH1 silencing, whereas, overexpression of ARIH1 leads to a reduction in hnRNP-E1. five9 backgroundWebTo examine the regulation of Cdc25A protein stability by USP29, we monitored Cdc25A expression in HEK293 cells cultured in the presence of an increasing concentration of USP29. ... In the cycloheximide chase experiment, we analyzed Cdc25A protein turnover in the presence and absence of USP29. ... For the immunoprecipitation assay, HEK293 … can indifference make the world greenerWebMay 30, 2024 · Cycloheximide Diterpenes / pharmacology* Epoxy Compounds / pharmacology Humans Male Phenanthrenes / pharmacology* Prostatic Neoplasms / drug therapy* Protein Stability / drug effects* Receptors, Androgen / drug effects* Antineoplastic Agents, Alkylating Diterpenes Epoxy Compounds Phenanthrenes Receptors, Androgen … can indifference curves have kinks